LOCUS       sequence-94696-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SIM2_pCSdest
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggct ccgcggcgcc cacccttcac catgaaggag
      181 aagtccaaga atgcggccaa gaccaggagg gagaaggaaa atggcgagtt ttacgagctt
      241 gccaagctgc tcccgctgcc gtcggccatc acttcgcagc tggacaaagc gtccatcatc
      301 cgcctcacca cgagctacct gaagatgcgc gccgtcttcc ccgaaggttt aggagacgcg
      361 tggggacagc cgagccgcgc cgggcccctg gacggcgtcg ccaaggagct gggatcgcac
      421 ttgctgcaga ctttggatgg atttgttttt gtggtagcat ctgatggcaa aatcatgtat
      481 atatccgaga ccgcttctgt ccatttaggc ttatcccagg tggagctcac gggcaacagt
      541 atttatgaat acatccatcc ttctgaccac gatgagatga ccgctgtcct cacggcccac
      601 cagccgctgc accaccacct gctccaagag tatgagatag agaggtcgtt ctttcttcga
      661 atgaaatgtg tcttggcgaa aaggaacgcg ggcctgacct gcagcggata caaggtcatc
      721 cactgcagtg gctacttgaa gatcaggcag tatatgctgg acatgtccct gtacgactcc
      781 tgctaccaga
//
