LOCUS       sequence-94719-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CLDN8_pCSdest
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcaac ccatgcctta gaaatcgctg
      181 ggctgtttct tggtggtgtt ggaatggtgg gcacagtggc tgtcactgtc atgcctcagt
      241 ggagagtgtc ggccttcatt gaaaacaaca tcgtggtttt tgaaaacttc tgggaaggac
      301 tgtggatgaa ttgcgtgagg caggctaaca tcaggatgca gtgcaaaatc tatgattccc
      361 tgctggctct ttctccggac ctacaggcag ccagaggact gatgtgtgct gcttccgtga
      421 tgtccttctt ggctttcatg atggccatcc ttggcatgaa atgcaccagg tgcacggggg
      481 acaatgagaa ggtgaaggct cacattctgc tgacggctgg aatcatcttc atcatcacgg
      541 gcatggtggt gctcatccct gtgagctggg ttgccaatgc catcatcaga gatttctata
      601 actcaatagt gaatgttgcc caaaaacgtg agcttggaga agctctctac ttaggatgga
      661 ccacggcact ggtgctgatt gttggaggag ctctgttctg ctgcgttttt tgttgcaacg
      721 aaaagagcag tagctacaga tactcgatac cttcccatcg cacaacccaa aaaagttatc
      781 acaccggaaa gaagtcaccg agcgtctact ccagaagtca gtatgtgtag
//
