LOCUS       sequence-9473-ex         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 3-G6HTnos-2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     295..315
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             356..373
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      393..645
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(644..743)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 caacaggatc gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa
       61 gttggaatac aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg
      121 catcaaagcc aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga
      181 tcattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      241 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      301 tcttgagttt gtaacagctg ctgggattac acatggcatg gtagaactat acaaacacca
      361 ccaccaccac cactaagaac tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
      421 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
      481 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
      541 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
      601 actaggataa attatcgcgc gcggtgtcat ctatgttact gatacccagc tttcttgtac
      661 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      721 agtcaaaata aaatcattat ttgccatcca gctgcagggc
//
