LOCUS       sequence-94730-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: KCNJ6_pCSdest
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggccaa gctgacagaa tccatgacta
      181 acgtcctgga gggcgactcc atggatcagg acgtcgaaag cccagtggcc attcaccagc
      241 caaagttgcc taagcaggcc agggatgacc tgccaagaca catcagccga gatcggacca
      301 aaaggaaaat ccagaggtac gtgaggaaag acggaaagtg caatgttcat cacggcaacg
      361 tgagggagac ctatcgctac ctgaccgata tcttcaccac attagtggac ctgaagtgga
      421 gattcaacct attgattttt gtcatggttt acacagtgac ctggctcttt tttggaatga
      481 tctggtggtt gatcgcatac atacggggag acatggacca catagaggac ccctcctgga
      541 ctccttgtgt taccaacctc aacgggttcg tctctgcttt tttattctca atagagacag
      601 aaaccaccat tggttatggc taccgggtca tcacagataa atgcccggag ggaattattc
      661 ttctcttaat ccaatctgtg ttggggtcca ttgtcaatgc attcatggtg ggatgcatgt
      721 ttgtaaaaat ctctcaaccc aagaagaggg cagagaccct ggtcttttcc acccatgcag
      781 tgatctccat gcgggatggg aaactgtgcc tgatgttccg ggta
//
