LOCUS       sequence-94734-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: USP25_pCSdest
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
      121 gatcacaagt ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tgaccgtgga
      181 gcagaacgtg ctgcagcaga gcgcggcgca gaagcaccag cagacgtttt tgaatcaact
      241 gagagaaatt acggggatta atgacaccca gatactacag caagccttga aggatagtaa
      301 tggaaacttg gaattagcag tggctttcct tactgcgaag aatgctaaga cccctcagca
      361 ggaggagaca acttactacc aaacagcact tcctggcaat gatagataca tcagtgtggg
      421 aagccaagca gatacaaatg tgattgatct cactggagat gataaagatg atcttcagag
      481 agcaattgcc ttgagtttgg ccgaatcaaa cagggcattc agggagactg gaataactga
      541 tgaggaacaa gccattagca gagttcttga agccagcata gcagagaata aagcatgttt
      601 gaagaggaca cctacagaag tttggaggga ttctcgaaac ccttatgata gaaaaagaca
      661 ggacaaagct cccgttgggc taaagaatgt tggcaatact tgttggttta gtgctgttat
      721 tcagtcatta tttaatcttt tggaatttag aagattagtt ctgaattaca agcctccatc
      781 aaatgctcaa gatttacccc gaaaccaaaa ggaacatcgg aatttgcctt ttatgcgtga
      841 gctgaggtat ctatttgcac ttcttgttgg taccaaaa
//
