LOCUS       sequence-94737-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0176
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..713
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(169..187)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(219..239)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     437..456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     631..654
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtcgacggat cggtgacggt gctggtttaa ttaacatggt ggcctcctcc gaggacgtca
       61 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
      121 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      181 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
      241 gctccaaggc gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
      301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      361 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagttc cgcggcacca
      421 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
      481 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
      541 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
      601 agctgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
      661 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
      721 gcaccggcag caccggcagc
//
