LOCUS       sequence-94738-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BACH1_pCSdest
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgtctctga
      181 gtgagaactc ggtttttgcc tatgaatctt ctgtgcatag caccaatgtt ttactcagcc
      241 ttaatgacca gcggaagaaa gatgtgctgt gcgatgtcac catctttgtg gagggacagc
      301 ggttccgcgc tcaccggtcc gtgctggcgg catgcagcag ttacttccac tcaagaatcg
      361 taggccaggc tgatggagag ctgaacatta ctcttccaga agaggtgaca gttaaaggat
      421 ttgaaccttt aattcagttt gcctacactg ctaaactgat tttaagtaaa gagaatgtgg
      481 atgaagtgtg caaatgtgtg gagtttttaa gtgtacataa tattgaggaa tcctgctttc
      541 agtttctgaa atttaagttt ttggactcca ctgcagacca gcaagaatgc ccaagaaaaa
      601 aatgcttttc atcacactgt cagaaaacag accttaaact ttcacttttg gaccagaggg
      661 atctagaaac tgatgaagtg gaggaatttc tggaaaataa aaatgttcag actcctcagt
      721 gtaaactccg caggtatcaa ggaaatgcaa aagcctcacc tcctctacaa gacagtgcca
      781 gtcagacata tgagtccatg tgcttagaga aggatgctgc tctgg
//
