LOCUS       sequence-94739-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0178
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(167..185)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(217..237)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     629..652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cgacggatcg gtgacggtgc tggtttaatt aacatggtgg cctcctccga ggacgtcatc
       61 aaagagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      181 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc
      241 tccaaggcgt acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      361 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagttccg cggcaccaac
      421 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
      481 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
      541 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcag
      601 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct ggggcatggc
      721 accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacgt catcaaagag
//
