LOCUS       sequence-94740-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MIS18A_pCSdest
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcagg cgttcggtca ctgaggtgta
      181 gcagaggatg cgctggcggc tgtgagtgcg gcgacaaggg caaatgcagc gactcctcgc
      241 tgttgggcaa gagactctcc gaagactcga gccgccacca gctgttgcag aagtgggcga
      301 gcatgtggag ctccatgagc gaagacgcgt cggtggccga catggagagg gcgcagctgg
      361 aggaggaggc ggcggctgcg gaggagaggc cgctggtgtt cctgtgctcc ggctgccggc
      421 ggccgctggg cgactcgctg agctgggtgg ccagccagga ggacaccaac tgcatcctgc
      481 ttcgctgtgt ttcctgtaat gtttctgtgg ataaggaaca gaagctatcc aaacgtgaaa
      541 aggaaaatgg ttgcgtcctt gagactttgt gctgcgcggg gtgctcactc aatcttggct
      601 acgtgtacag atgcacgccc aagaatcttg attacaagag agacttgttt tgcctcagtg
      661 ttgaagccat tgaaagttat gttttagggt cctctgaaaa gcaaattgtg tcagaagata
      721 aagagctttt taatcttgaa agcagagttg aaatagaaaa gtctctaaca cagatggaag
      781 atgtcttgaa agcattacaa atgaagctgt
//
