LOCUS       sequence-94752-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st2):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1853
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..636)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     544..563
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(780..807)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tacgattaaa gcttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
       61 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
      121 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
      181 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
      241 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
      301 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
      361 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
      421 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
      481 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
      541 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
      601 gctgttaatc actttacttt tatctaatct agacattttc ctccttaccc gggtgtgtgg
      661 aatccattat aaccgccaag cataaaaatg tcaacaacga cggtgctcga ggatcctgag
      721 cggatacata tttgaatgta tttagaaaaa taaacaaaaa gagtttgtag aaaacgcaaa
      781 aaaggccatc cgtcaggatg gccttctgct taaatttgat
//
