LOCUS       sequence-94759-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:RBS(st7):gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1801
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..734)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(61..81)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     676..704
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    763..779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccctgtacga ttaaagctta tttgtatagt tcatccatgc catgtgtaat cccagcagct
       61 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      121 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      181 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      241 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      301 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      361 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      421 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      481 ttgaaaaagt catgctgttt catatgatct gggtatctcg caaagcattg aacaccataa
      541 ccgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      601 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      661 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct
      721 tctcctttac gcattttttt ttcctcctta cccgggagta tcttgttatc cgctcacaa
//
