LOCUS       sequence-94763-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR4_pCSdest
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             3..17
                     /estimated_length=15
     promoter        73..91
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     73..90
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    145..169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(530..554)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    611..732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(622..641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     676..695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccnnnnnnnn nnnnnnntag catgctcgtt tagggaaccg ccattctgcc tggggacgtc
       61 ggagcaagct tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat
      121 cccatcgatt cgaattcaag gatcacaagt ttgtacaaaa aagcaggcac catgtcgtta
      181 atcatcttga cgagagatga tgaaccccgg atatttaccc cagacagtga tgccgcttca
      241 ccagcattgc actctacttc cccgcttcct gatcctgcct cagcttctcc tctccacaga
      301 gaagaaaaaa ttctgcctaa agtctgcaac atcgtttcct gcctgagttt cagcctgcca
      361 gcttctccta cggattctgg acttgccagc cccacaatca taaccagaga ggggcagcaa
      421 ttttgggcaa aatgtctgat ttggaaatac caactttacc tccatgggct ccacaagaaa
      481 tcagatggga gaagggacaa gcagataagc gcaagcccat caacctagaa cccagctttc
      541 ttgtacaaag tggtgatcct ctcgagcctc tagaactata gtgagtcgta ttacgtanat
      601 ccagacatga taagatacat tgatgagttt ggacaaacca caactanaat gcantgaaaa
      661 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      721 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcangttca nggggaggtg
      781 tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggnccggta cccagctttt
      841 gttcccttta gtgagggtta nttgcgcgct tggcgtaatc atggncatan ctgtttcctg
      901 tgtgaattgt tatccgctca nattccacac aacatacnag ccgggancat aagtgnaann
      961 cctggggnnc ntnanngant gagctaactc acattannng ngttgcgctt cactgcncnn
     1021 ctttnnnagg t
//
