LOCUS       sequence-94784-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full-length Secreted protein acidic and rich in cysteine 
            precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag,
            biotinylation sequence and His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: SPARC-bio-His
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataaatttc ctttattagc cagaggtcga ggtcggggga tccttaatta atcacgtgtc
       61 tagactcaat ggtggtgatg gtggtggggg cccagagggg ccagaggagg caggttcctg
      121 tccctgtggt tccacagcat cttctgggcg tccaggatgt ggtgcaggct gccggaattc
      181 aaccctttgg ataaaacctg gatcttggag tccatcttga cctcttcacc ttcactcagt
      241 agacattgcc acacccctgc ttcaggggcc tgcacttgaa tcactttctc ctgcctggag
      301 accctggcct cctgattctc ctgcttcaag atcagtctca tcttgggtga ggtgggtccc
      361 atcacctcac aggtcaaagt gttgctgtcg ggctgagtca ctttcatcac caccaggttc
      421 acttcctgat acagtatccc tctgtccaga gtcagggtca ggttgccaga accagcaaac
      481 tgaagggaga cctggggtat ctgaagggtg agtgggagcg tttcggacag ctggaacttg
      541 gggttgctag tagacttctg cacagacacc ttttggttct ttagggagaa ggtgatccag
      601 gactgggaag aaggagcctt ctctgccttc catctcaact ctccctgcag gctttcctct
      661 ccaaggttga gtgggaagga gaactccgct gactccccct cactcttata ggccgtgatg
      721 gaggtcgacg gcgcgccaat gaccaggtcc ttgtcgatgt ccttctgctt gatgccgaag
      781 cagccggccc actcgtccag ggcaatgtac ttgtcgttgt cc
//
