LOCUS       sequence-94797-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TMEM50B_pCSdest
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(628..652)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(720..739)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     774..793
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaagga
      121 tcacaagttt gtacaaaaaa gcaggcacca tggcaggctt cctagataat tttcgttggc
      181 cagaatgtga atgtattgac tggagtgaga gaagaaatgc tgtggcatct gttgtcgcag
      241 gtatattgtt ttttacaggc tggtggataa tgattgatgc agctgtggtg tatcctaagc
      301 cagaacagtt gaaccatgcc tttcacacat gtggtgtatt ttccacattg gctttcttca
      361 tgataaatgc tgtatccaat gctcaggtga gaggtgatag ctatgaaagc ggctgtttag
      421 gaagaacagg tgctcgagtt tggcttttca ttggtttcat gttgatgttt gggtcactta
      481 ttgcttccat gtggattctt tttggtgcat atgttaccca aaatactgat gtttatccgg
      541 gactagctgt gttttttcaa aatgcactta tattttttag cactctgatc tacaaatttg
      601 gaagaaccga agagctatgg acctagaacc cagctttctt gtacaaagtg gtgatcctct
      661 cgagcctcta gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg
      721 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      781 gtgatgctat tgctttattt gtaaccatta taagctgcaa
//
