LOCUS       sequence-94815-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: N6AMT1_pCSdest
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(715..739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcagg ggagaacttc gctacgccgt
      181 tccacgggca cgtgggccgc ggcgccttca gcgacgtgta cgagcccgcg gaggacacgt
      241 ttctgctttt ggacgcgctc gaggcagcgg ctgccgaact ggcaggagtg gaaatatgcc
      301 tggaagtagg gtcagggtct ggtgtagtat ctgcattcct agcctctatg ataggccctc
      361 aggctttgta catgtgcact gatatcaacc ctgaggcagc agcttgtacc ctagagacag
      421 cacgctgtaa caaagttcac attcaaccag ttattacaga tttggtagga agtcacggaa
      481 tagaggcagc ttgggctggt ggcagaaatg gtcgggaagt catggacagg ttttttcccc
      541 tggttccaga tctcctttca ccaagaggat tattctattt agttaccatt aaagaaaaca
      601 acccagaaga aattttgaaa ataatgaaga caaaaggtct gcaaggaacc actgcacttt
      661 ccagacaagc aggccaagaa actctttcag tcctcaagtt caccaagtct tagaacccag
      721 ctttcttgta caaagtggtg atcctctcga gcctctagaa ctatagtgag tcgtattacg
      781 tagatccaga catgataaga tacattgatg agtttggac
//
