LOCUS       sequence-94817-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CLDN17_pCSdest
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             508..519
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcatt ttatcccttg caaattgctg
      181 ggctggttct tgggttcctt ggcatggtgg ggactcttgc cacaaccctt ctgcctcagt
      241 ggagagtatc agcttttgtt ggcagcaaca ttattgtctt tgagaggctc tgggaagggc
      301 tctggatgaa ttgcatccga caagccaggg tccggttgca atgcaagttc tatagctcct
      361 tgttggctct cccgcctgcc ctggaaacag cccgggccct catgtgtgtg gctgttgctc
      421 tctccttgat cgccctgctt attggcatct gtggcatgaa gcaggtccag tgcacaggct
      481 ctaacgagag ggccaaagca taccttctgg gaacttcagg agtcctcttc atcctgacgg
      541 gcatcttcgt tctgattccg gtgagctgga cagccaatat aatcatcaga gatttctaca
      601 acccagccat ccacataggt cagaaacgag agctgggagc agcacttttc cttggctggg
      661 caagcgctgc tgtcctcttc attggagggg gtctgctttg tggattttgc tgctgcaaca
      721 gaaagaagca agggtacaga tatccagtgc ctggctaccg tgtgccacac acagat
//
