LOCUS       sequence-94819-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf119_pCSdest
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(339..363)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    407..541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(431..450)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     485..504
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(654..674)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(654..672)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(693..709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(706..728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    717..733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(741..747,748..765,766..771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    786..807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcatgctcgt ttagggaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
      121 gatcacaagt ttgtacaaaa aagcaggcac catggggcga gcggacactc cacgacaccc
      181 cccgcccccg gctgcagggt ttggggtcca ccggggcgca tttctgatcc cagtggctct
      241 tcgggtgctc ttggccggaa gaactcccag acctttcact cctggcctgg ccgatccacg
      301 aagactcggg ccccggcgcg tccaagcagc acaatagaac ccagctttct tgtacaaagt
      361 ggtgatcctc tcgagcctct agaactatag tgagtcgtat tacgtagatc cagacatgat
      421 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      481 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      541 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      601 ttaattcgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg ttccctttag
      661 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      721 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
      781 gcctaatgag tgagctaact cacattaatt gcg
//
