LOCUS       sequence-94830-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CBR3_pCSdest
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggatcacaa gtttgtacaa aaaagcaggc accatgtcgt cctgcagccg cgtggcgctg
      181 gtgaccgggg ccaacagggg catcggcttg gccatcgcgc gcgaactgtg ccgacagttc
      241 tctggggatg tggtgctcac cgcgcgggac gtggcgcggg gccaggcggc cgtgcagcag
      301 ctgcaggcgg agggcctgag cccgcgcttc caccaactgg acatcgacga cttgcagagc
      361 atccgcgccc tgcgcgactt cctgcgcaag gagtacgggg ggctcaatgt actggtcaac
      421 aacgcggccg tcgccttcaa gagtgatgat ccaatgccct ttgacattaa agctgagatg
      481 acactgaaga caaatttttt tgccactaga aacatgtgca acgagttact gccgataatg
      541 aaacctcatg ggagagtggt gaatatcagt agtttgcagt gtttaagggc ttttgaaaac
      601 tgcagtgaag atctgcagga aaggttccac agtgagacac tcacagaagg agacctggtg
      661 gatctcatga aaaagtttgt ggaggacaca aaaaatgagg tgcatgagag ggaaggctgg
      721 cccaactcac cttatggggt gtccaagttg ggggtcacag tcttatcgag gat
//
