LOCUS       sequence-94850-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses full-length Proteinase-activated receptor 1 precursor 
            ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, 
            biotinylation sequence and His tag. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: F2R-bio-His
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataaatttcc tttattagcc agaggtcgag gtcgggggat ccttaattaa tcacgtgtct
       61 agactcaatg gtggtgatgg tggtgggggc ccagaggggc cagaggaggc aggttcctgt
      121 ccctgtggtt ccacagcatc ttctgggcgt ccaggatgtg gtgcaggctg ccggaattca
      181 accctttgga taaaacctgg atcttggagt ccatcttgac ctcttcacct tcactcagta
      241 gacattgcca cacccctgct tcaggggcct gcacttgaat cactttctcc tgcctggaga
      301 ccctggcctc ctgattctcc tgcttcaaga tcagtctcat cttgggtgag gtgggtccca
      361 tcacctcaca ggtcaaagtg ttgctgtcgg gctgagtcac tttcatcacc accaggttca
      421 cttcctgata cagtatccct ctgtccagag tcagggtcag gttgccagaa ccagcaaact
      481 gaagggagac ctggggtatc tgaagggtga gtgggagcgt ttcggacagc tggaacttgg
      541 ggttgctagt agacttctgc acagacacct tttggttctt tagggagaag gtgatccagg
      601 actgggaaga aggagccttc tctgccttcc atctcaactc tccctgcagg ctttcctctc
      661 caaggttgag tgggaaggag aactccgctg actccccctc actcttatag gccgtgatgg
      721 aggtcgacgg cgcgccgggc acgaacaggg tcagccagct gctggtcagg tagccgctgg
      781 cgtcctcgct gatgaaggcg ggcagctgct tctgca
//
