LOCUS       sequence-94877-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TMPRSS3_pCSdest
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggat
      121 cacaagtttg tacaaaaaag caggcaccat gggggaaaat gatccgcctg ctgttgaagc
      181 ccccttctca ttccgatcgc tttttggcct tgatgatttg aaaataagtc ctgttgcacc
      241 agatgcagat gctgttgctg cacagatcct gtcactgctg ccattgaagt tttttccaat
      301 catcgtcatt gggatcattg cattgatatt agcactggcc attggtctgg gcatccactt
      361 cgactgctca gggaagtaca gatgtcgctc atcctttaag tgtatcgagc tgatagctcg
      421 atgtgacgga gtctcggatt gcaaagacgg ggaggacgag taccgctgtg tccgggtggg
      481 tggtcagaat gccgtgctcc aggtgttcac agctgcttcg tggaagacca tgtgctccga
      541 tgactggaag ggtcactacg caaatgttgc ctgtgcccaa ctgggtttcc caagctatgt
      601 gagttcagat aacctcagag tgagctcgct ggaggggcag ttccgggagg agtttgtgtc
      661 catcgatcac ctcttgccag atgacaaggt gactgcatta caccactcag tatatgtgag
      721 ggagggatgt gcctctggcc acgtggttac cttgcagtgc acagcctgtg gtcatag
//
