LOCUS       sequence-94882-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ATP5J_pCSdest
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(481..505)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    549..683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(573..592)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     627..646
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(796..816)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(796..814)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgattct tcagaggctc ttcaggttct
      181 cctctgtcat tcggtcagcc gtctcagtcc atttgcggag gaacattggt gttacagcag
      241 tggcatttaa taaggaactt gatcctatac agaaactctt tgtggacaag attagagaat
      301 acaaatctaa gcgacagaca tctggaggac ctgttgatgc tagttcagag tatcagcaag
      361 agctggagag ggagcttttt aagctcaagc aaatgtttgg taatgcagac atgaatacat
      421 ttcccacctt caaatttgaa gatcccaaat ttgaagtcat cgaaaaaccc caggcctaga
      481 acccagcttt cttgtacaaa gtggtgatcc tctcgagcct ctagaactat agtgagtcgt
      541 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      601 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      661 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      721 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
      781 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      841 gctg
//
