LOCUS       sequence-94883-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: LTN1_pCSdest
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gacccacgcg
      181 tccgaaccgg ggtctgagtg ctcagagtac agctgcaacc gcgaccatgg gcgggaagaa
      241 caagcagcga actaaaggga acctgaggcc ttcaaacagt ggccgagctg cagaactcct
      301 tgccaaagaa cagggaacag tgcctggatt tattggtttt ggaacatctc agagtgacct
      361 aggctatgtt cctgctattc aaggagctga agaaattgac agtcttgtag attctgattt
      421 ccgaatggtg ctgcggaaac tttcaaagaa agatgtcacc acaaaattaa aagctatgca
      481 ggaatttgga accatgtgta cagagagaga cacagaaact gtgaaaggag ttcttccata
      541 ttggccaaga attttttgca aaatttcact tgatcatgac cgtcgcgtcc gagaagccac
      601 acaacaagct tttgaaaaac ttatccttaa agtaaagaaa cagttggctc cctacttaaa
      661 aagtttaatg ggatattggc taatggctca gtgtgatact tacacaccag ctgcgtttgc
      721 agcaaaagat gcatttgaag cggcttttcc tccaagcaag caacctgaag ccatagcatt
      781 ttgtaaggat gaaattacaa gtgtgctgca
//
