LOCUS       sequence-94895-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: Dopey2_pCSdest
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggat
      121 cacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgg tgacagtccc
      181 tcccattgct cctgggatac tgaaagtcat tgccgagaaa acagacttct atcagagggt
      241 ggctcgtgtg ctctggaacc agctgaacaa ggagacccgt gaacatcaca tcacctgtgt
      301 agagctgttc taccgcctgc actgcctggc cccgactgcc aacatctgtg aggatatcat
      361 ctgccatgcc ctcctggacc ctgacaaggg aacaaggttg gaagctctgt ttaggttttc
      421 tgtgatctgg cacctgacac gagaaatcca aggtagtcgt gtgacgtccc acaatcgctc
      481 ctttgacagg tccttgtttg tcgtcctgga tagcctggct tgcacagacg gtgctatcag
      541 tgcagcagcc cagggctggc tggtgcgagc gctctccctc ggggatgtgg cccgcatcct
      601 cgaacccatg ctcctgctgt tgctccagcc caaaacccag aggacgtcca tccagtgtct
      661 caagcaggag aactcagccg aggatttaca ccgttgg
//
