LOCUS       sequence-94912-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-EGFP-L
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      202..211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(353..371)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     621..640
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gattaagcgt ctgatgagtc cgtgaggacg aaacccggcg taccgggtca
      121 cgcttaacaa ccagatcaaa gaaaaaacag acattgtcaa ttgcaaagca aaaatgtaac
      181 acccctacac cggtgaaagc cgccaccatg gtgagcaagg gcgaggagga taacatggcc
      241 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      301 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      361 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      421 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      481 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      541 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      601 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      661 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      721 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      781 gtgcagctgc ccggcg
//
