LOCUS       sequence-94918-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CHAF1B_pCSdest
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgaaagt catcacttgt gaaatagcct
      181 ggcacaacaa ggagcccgtg tacagcctgg acttccagca tgggacggct gggaggatcc
      241 acagactggc gtctgccggc gtggacacca atgtcaggat ctggaaggta gaaaagggac
      301 cagatggaaa agccatcgtg gaatttttgt ccaatcttgc tcgtcatacc aaagccgtca
      361 atgttgtgcg tttttctcca actggggaaa ttttagcatc gggaggagat gatgctgtca
      421 tcctattgtg gaaggtgaat gataacaagg agccggagca gatcgctttt caggatgagg
      481 acgaggccca gctgaacaag gagaactgga cggttgtgaa gactctgcgg ggccacttag
      541 aagatgtgta tgatatttgc tgggcaactg atgggaattt aatggcttct gcctctgtgg
      601 ataacacagc catcatatgg gatgtcagca aaggacaaaa gatatcaatt tttaatgaac
      661 ataaaagtta tgtccaagga gtaacctggg accctttggg tcaatatgtt gctactctga
      721 gctgtgacag ggtgctgcga gtatacagta tacag
//
