LOCUS       sequence-94920-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR9_pCSdest
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(605..629)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(697..716)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggatcacaa gtttgtacaa aaaagcaggc accatgggca ggatttgccc cgtgaactcc
      181 cgcgcacgca ggctccgcgc gaggcccggg cgcccgagcg gcgattccct cccctatcac
      241 cagctccagg gtggtgcccc ccggctgtgg agcccagacc ccggccgtcc agcagcgtat
      301 agacgagccc atgtctgcga tgtaactgca ccgcgctggg gatccacctc ccgccagggt
      361 gagggcgccg tcctccagct tatgctaggc aggcgcgccc cgccctcctg gtcacgtgac
      421 tacgcctaca gccgaagagg atgggaaaat gcggccctgt tcctgaacag gaagcggaag
      481 caggaaggaa ctgagaacac cagcatctgc tgccgccctg aatcagcgtt agcatgtgga
      541 ggcaacctca gcccccagtt tttgaagaag gtgattcaga ttcagacaca ggaattatgg
      601 tagaacccag ctttcttgta caaagtggtg atcctctcga gcctctagaa ctatagtgag
      661 tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca aaccacaact
      721 agaatgcagt g
//
