LOCUS       sequence-94926-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: USP16_pCSdest
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgggaaa gaaacggaca aagggaaaaa
      181 ctgttccaat cgatgattcc tctgaaactt tagaacctgt gtgcagacac attagaaaag
      241 gattggaaca aggtaatttg aaaaaggctt tagtgaatgt ggaatggaat atctgccaag
      301 actgtaagac tgacaataaa gtgaaagata aagctgaaga agaaacagaa gaaaagcctt
      361 cagtttggct gtgtcttaaa tgtggccatc agggctgtgg cagaaattct caggagcagc
      421 atgccttgaa gcactatctg acgccaagat ctgaacctca ctgtctggtt cttagtttgg
      481 acaactggag tgtatggtgt tacgtatgtg ataatgaggt ccagtattgt agttcaaacc
      541 agttgggtca agtggttgat tatgtcagaa aacatgccag cattacaact ccaaagccag
      601 agaaagataa tggaaatatt gaacttgaaa ataaaaaatt agaaaaagag agtaagaatg
      661 aacaagagag agaaaagaag gaaaacatgg ctaaagagaa tcctcccatg aattctcctt
      721 gccaaataac cgtgaaagga ctcagtaatt tgggaaacac atgtttcttc aatgcagtta
      781 tgcagaactt gtcacaaaca ccagtgctta gagaactact
//
