LOCUS       sequence-94927-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SOD1_pCSdest
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(615..639)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(707..726)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggat
      121 cacaagtttg tacaaaaaag caggcaccat ggcgacgaag gccgtgtgcg tgctgaaggg
      181 cgacggccca gtgcagggca tcatcaattt cgagcagaag gaaagtaatg gaccagtgaa
      241 ggtgtgggga agcattaaag gactgactga aggcctgcat ggattccatg ttcatgagtt
      301 tggagataat acagcaggct gtaccagtgc aggtcctcac tttaatcctc tatccagaaa
      361 acacggtggg ccaaaggatg aagagaggca tgttggagac ttgggcaatg tgactgctga
      421 caaagatggt gtggccgatg tgtctattga agattctgtg atctcactct caggagacca
      481 ttgcatcatt ggccgcacac tggtggtcca tgaaaaagca gatgacttgg gcaaaggtgg
      541 aaatgaagaa agtacaaaga caggaaacgc tggaagtcgt ttggcttgtg gtgtaattgg
      601 gatcgcccaa tagaacccag ctttcttgta caaagtggtg atcctctcga gcctctagaa
      661 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
      721 aaccacaact ag
//
