LOCUS       sequence-94930-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of dsRed and constitutive expression of GFP in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW
  TITLE     Dynamics of intracellular bacterial replication at the single cell 
            level.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 
            10.1073/pnas.1000041107. Epub 2010 Feb 2.
  PUBMED    20133586
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1000041107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-02-23- 23"; volume: "107"; issue: "8"; pages: "3746-51"
COMMENT     Sequence Label: pDiGc
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(233..251)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(283..303)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     501..520
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     695..718
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
       61 tggtacccgg ggatcctcta gatttaagaa ggagatatac atatggcctc caccgaggac
      121 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag
      181 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      241 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
      301 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
      361 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      421 gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc
      481 gtgaacttcc cctccgacgg ccccgtaatg cagaagaaga ctatgggctg ggagccctcc
      541 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggccctgaag
      601 ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc
      661 gtgcagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag
      721 gactacacca tcgtgga
//
