LOCUS       sequence-94931-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CRYZL1_pCSdest
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     50..67
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
       61 actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga attcaaggat
      121 cacaagtttg tacaaaaaag caggcaccat gaaaggctta tatttccaac agagttccac
      181 agatgaagaa ataacatttg tatttcaaga aaaggaagat cttcctgtta cagaggataa
      241 ctttgtgaaa cttcaagtta aagcttgtgc tctgagccag ataaatacaa agcttctggc
      301 agaaatgaag atgaaaaagg atttatttcc tgttgggaga gaaattgctg gaattgtatt
      361 agatgttgga agcaaggtat cattctttca accagatgat gaagtagttg gaattttgcc
      421 cctggactct gaagaccctg gactttgtga agttgttaga gtacatgagc attacttggt
      481 tcataaacca gaaaaggtca catggacgga agcagcagga agcattcggg atggagtgcg
      541 tgcctataca gctctgcatt atctttctca tctctctcct ggaaaatcag tgctgataat
      601 ggatggagca agtgcatttg gtacaatagc tattcagtta gcacatcata gaggagccaa
      661 agtgatttca acagcatgca gccttgaaga taagcagtgc cttgaaagat tcagacctcc
      721 catagcccga gtgattgatg tatctaat
//
