LOCUS       sequence-94932-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: RCAN1_pCSdest
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaagga
      121 tcacaagttt gtacaaaaaa gcaggcacca tggaggaggt ggacctgcag gacctgccca
      181 gcgccaccat cgcctgtcac ctggacccgc gcgtgttcgt ggacggcctg tgccgggcca
      241 aatttgagtc cctctttagg acgtatgaca aggacatcac ctttcagtat tttaagagct
      301 tcaaacgagt cagaataaac ttcagcaacc ccttctccgc agcagatgcc aggctccagc
      361 tgcataagac tgagtttctg ggaaaggaaa tgaagttata ttttgctcag accttacaca
      421 taggaagctc acacctggct ccgccaaatc cagacaagca gtttctgatc tcccctcccg
      481 cctctccgcc agtgggatgg aaacaagtgg aagatgcgac cccagtcata aactatgatc
      541 tcttatatgc catctccaag ctggggccag gggaaaagta tgaattgcac gcagcgactg
      601 acaccactcc cagcgtggtg gtccatgtat gtgagagtga tcaagagaag gaggaagaag
      661 aggaaatgga aagaatgagg agacctaagc caaaaattat ccagaccagg aggccggagt
      721 acacgccgat ccacctcagc tagaacccag ctttcttgta caaagt
//
