LOCUS       sequence-94935-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of GFP and constitutive expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Figueira R, Watson KG, Holden DW, Helaine S
  TITLE     Identification of salmonella pathogenicity island-2 type III 
            secretion system effectors involved in intramacrophage replication 
            of S. enterica serovar typhimurium: implications for rational 
            vaccine design.
  JOURNAL   MBio. 2013 Apr 16;4(2):e00065. doi: 10.1128/mBio.00065-13.
  PUBMED    23592259
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2013-04-16"; volume: "4(2):e00065. doi"; pages: " 
            10.1128/mBio.00065-13"
COMMENT     Sequence Label: pFCcGi
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(133..161)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     756..776
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
       61 tggtacccgg ggatcctcta gatttaagaa ggagatatac atatgagtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaaca
//
