LOCUS       sequence-94937-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of dsRed and GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW
  TITLE     Dynamics of intracellular bacterial replication at the single cell 
            level.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 
            10.1073/pnas.1000041107. Epub 2010 Feb 2.
  PUBMED    20133586
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1000041107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-02-23- 23"; volume: "107"; issue: "8"; pages: "3746-51"
COMMENT     Sequence Label: pDiGi
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(118..146)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgaattcc cggattaatt aagtgggatc
       61 ctctagattt aagaaggaga tatacatatg agtaaaggag aagaactttt cactggagtt
      121 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      181 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      241 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      301 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      361 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      421 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      481 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      541 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      601 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      661 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccct
//
