LOCUS       sequence-94939-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to make YFP-Parkin stable cell line with retrovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Yamano K, Fogel AI, Wang C, van der Bliek AM, Youle RJ
  TITLE     Mitochondrial Rab GAPs govern autophagosome biogenesis during 
            mitophagy.
  JOURNAL   Elife (Cambridge). 2014 Feb 25;3:e01612. doi: 10.7554/eLife.01612.
  PUBMED    24569479
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 
            (Cambridge)."; date: "2014-02-25"; volume: "3:e01612. doi"; pages: "
            10.7554/eLife.01612"
COMMENT     Sequence Label: pBMN-YFP-Parkin
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(39..41,42..44,45..755)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(84..105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(345..364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     692..713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgacccgggg gtggaccatc ctctagactg ccggatccat ggtgagcaag ggcgaggagc
       61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      121 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
      241 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      481 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      541 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
      661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      721 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
      781 ctttgatagt gtttgtcagg ttcaactcca gccatggttt cccagtggag gtcgattctg
      841 acaccagcat cttccagctc
//
