LOCUS       sequence-94942-e         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active PRAS40 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Xiong X, Xie R, Zhang H, Gu L, Xie W, Cheng M, Jian Z, Kovacina K, 
            Zhao H
  TITLE     PRAS40 plays a pivotal role in protecting against stroke by linking 
            the Akt and mTOR pathways.
  JOURNAL   Neurobiol Dis. 2014 Jun;66:43-52. doi: 10.1016/j.nbd.2014.02.006. 
            Epub 2014 Feb 27.
  PUBMED    24583056
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis."; date: "2014-06"; volume: "66:43-52. doi"; pages: " 
            10.1016/j.nbd.2014.02.006. Epub 2014 Feb 27"
COMMENT     Sequence Label: pHRIG-PRAS40
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..154
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tttagtgaac cggtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
       61 gacaccgact ctagaggatc tcgagctcaa gcttcgaatt ctgcagcccg ggggatccac
      121 catggaacaa aagctcattt ctgaagaaga tctcggaatt ctcgcgtcgg ggcgccccga
      181 ggagctgtgg gaggccgtgg tgggggccgc ggagcgcttc cgggcccgga ctggcacgga
      241 gctggtgctg ctgaccgcgg ccccgccgcc accaccccgc ccgggcccct gtgcctatcc
      301 tgcccatggt cgaggagccc tggcggaggc agcgcgccgt tgcctccac
//
