LOCUS       sequence-94943-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of dominant negative mouse Akt1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-AktDN
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtgaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gactctagag gatctcgagc tcaagcttcg aattctgcag tcgaagcttc gatcgtcgac
      121 atgtacccat acgatgttcc agattacgct tctagaatga acgacgtagc cattgtgaag
      181 gagggctggc tgcacaaacg aggggaatat attaaaacct ggcggccacg ctacttcctc
      241 ctcaagaacg atggcacctt tattggctac aaggaacggc ctcaggatgt ggatcagcga
      301 gagtccccac tcaacaactt ctcagtggca caatgccagc tgatgaagac agagcggcca
      361 aggcccaaca cctttatcat ccgctgcctg cagtggacca cagtcattga gcgcaccttc
      421 catgtggaaa cgcctgagga gcgggaagaa tgggccaccg ccattcagac tgtggccgat
      481 ggactcaaga ggcaggaaga agagacgatg gacttccgat caggctcacc cagtgacaac
      541 tcaggggctg aagagatgga ggtgtccctg gccaagccca agcaccgtgt gaccatgaac
      601 gagtttgagt acctgaaact actgggcaag ggcacctttg ggaaagtgat tctggtgaaa
      661 gagaaggcca caggccgcta ctatgccatg atgatcctca agaaggaggt catcgtcgcc
      721 aaggatgagg ttgcccacac gcttactgag aaccgtgtcc tgcagaactc taggcatccc
      781 tttccttacg gccctcaagt actcattcca gacccacgac cgcctctgct ttgtcatgga
      841 gtatgcc
//
