LOCUS       sequence-94945-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt3 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt3
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..164
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgactctaga ggatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcca
      121 ccatggggag tagcaagagc aagcctaagg accccagcca gcgcagcgat ggagaggaag
      181 agatggatgc ctctacaacc catcataaaa gaaagacaat gaatgatttt gactatttga
      241 aactactagg taaaggcact tttgggaaag ttattttggt tcgagagaag gcaagtggaa
      301 aatactatgc tatgaagatt ctgaagaaag aagtcattat tgcaaaggat gaagtggcac
      361 acactctaac tgaaagcaga gtattaaaga acactagaca tcccttttta acatccttga
      421 aatattcctt ccagacaaaa gaccgtttgt gttttgtgat ggaatatgtt aatgggggcg
      481 agctgttttt ccatttgtcg agagagcggg tgttctctga ggaccgcaca cgtttctatg
      541 gtgcagaaat tgtctctgcc ttggactatc tacattccgg aaagattgtg taccgtgatc
      601 tcaagttgga gaatctaatg ctggacaaag atggccacat aaaaattaca gattttggac
      661 tt
//
