LOCUS       sequence-94946-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt1
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..182
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgcactctag aggatctcga gctcaagctt cgaattctgc agtcgacggt atcgataagc
      121 ttgatatcga attcgccgcc atggggagta gcaagagcaa gcctaaggac cccagccagc
      181 gcagcgaggc tgaagagatg gaggtgtccc tggccaagcc caagcaccgc gtgaccatga
      241 acgagtttga gtacctgaag ctgctgggca agggcacttt cggcaaggtg atcctggtga
      301 aggagaaggc cacaggccgc tactacgcca tgaagatcct caagaaggaa gtcatcgtgg
      361 ccaaggacga ggtggcccac acactcaccg agaaccgcgt cctgcagaac tccaggcacc
      421 ccttcctcac agccctgaag tactctttcc agacccacga ccgcctctgc tttgtcatgg
      481 agtacgccaa cgggggcgag ctgttcttcc acctgtcccg ggaacgtgtg ttctccgagg
      541 accgggcccg cttctatggc gctgagattg tgtcagccct ggactacctg cactcggaga
      601 agaacgtggt gtaccgggac ctcaagctgg agaacctcat gctggacaag gacgggcaca
      661 ttaagatcac agacttcggg ctgtgcaagg aggggatcaa ggacggtgcc accatgaaga
      721 ccttttgcgg cacacctgag tacctg
//
