LOCUS       sequence-94948-e          20 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. 
            Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Aug 6. doi: 10.1038/nature13589."
COMMENT     Sequence Label: pX330 Pten
FEATURES             Location/Qualifiers
     source          1..20
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agatcgttag cagaaacaaa
//
