LOCUS       sequence-94964-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: ATP5O_pCSdest
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(582..593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(704..728)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(782..800)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(783..800)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gactcactat agttctagag gctcgagagg atcaccactt tgtacaagaa agctgggttc
       61 tagacaatct cccgcatagc cctgcccagc ttctgaatct tggtcttgac agacatgtca
      121 acatatttct cgccaatgcg cacaatcatt ccacccaaga ttgacggatc agtcttagcc
      181 tccaatttca atacttggcc ttgacttagg aagctcttga ggacagtttt taattcagag
      241 agtgtggctt cttctaaagg agatgcagag gtcactgtgc aaggtacctc tccgcgatgg
      301 acactcatca tggtagaaaa ggcagaaacg actccttggg tattgcttaa tcgaccattt
      361 tcagcaagca aattgatcag gttggtagtg aggggagaga acctctcttt tgctgtgatg
      421 tcatttaggc ttttcacttt aatggaacgc ttcacatagg gattcaaaac agaagcagcc
      481 actttgggtt ccttcaggat ttgtgctact ctcaacaact ccttttctac ttgctccagc
      541 ttattctgtt ttgatgcagc agaataaaga gctgtggcat agcgaccttc aataccgtat
      601 acctgaacag gaggcctcac aagcttggca aatggtctga ccacagaggt actgaagcat
      661 cgcacctgcc gggagagccc ggacactgct ggggcagcca tggtgcctgc ttttttgtac
      721 aaacttgtga tccttgaatt cgaatcgatg ggatcctgca aaaagaacaa gtagcttgta
      781 ttctatagtg tcacctaaat caagcttgct ccgacgtccc caggcag
//
