LOCUS       sequence-94983-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: MRPS6_pCSdest
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(443..467)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(521..539)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(522..539)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctacttct tcctcttctt tgtggaatat aatttttctg cgagtgggac tgggacaatc
      121 ccttcacatt cttttagttc ctgggtcaga gggtgtttga caatattccc tctaatcaca
      181 tctatatctc gagacaagtg ctccaccatg ctttcaacag ctgcggtggg tgcataaaaa
      241 tccaccaaga aatacccgcc tctgttgtgc tgctgactgt gggcagagat cctataagga
      301 agcgctcgtt cacccaggtt ttccaagtcc ctcactattg ctcctctgtc catcagggcc
      361 tctatcgtac gtttcaaagt agcagcagtc tctggccgct gcatggcttt caggattaaa
      421 gccagctcgt agcggggcat ggtgcctgct tttttgtaca aacttgtgat ccttgaattc
      481 gaatcgatgg gatcctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatc
      541 aagcttgctc cgacgtcccc aggcagaatg gcggttccct aaacgagcat tgcttatata
      601 gacctcccat taggcacgcc taccgcccat ttacgtcaat ggaacgccca tttgcgtcat
      661 tgcccctccc cattgacgtc aatggggatg tacttggcag ccatcgcggg ccatttaccg
      721 ccattgacgt caatgggagt actgccaatg taccctggcg tacttccaat agtaatgtac
      781 ttgccaagtt actattaata gatattgatg tactg
//
