LOCUS       sequence-94988-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR6_pCSdest
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(638..662)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(716..734)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(717..734)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctatttgg atgaggagca cttgccaccc cttgaggaga caccttgagg gagcggaccc
      121 tccccccagt ggtcaccccc tcctgaggat cgctgccctt ggttgatgcc ctggtctctg
      181 ccctttcccc ctgggccatt ttcctgccga cctcccacct cctggggcag aagatggagg
      241 ggtgggggtc cttcctctgg ctcttcagct tcggaagcag actcagtaga ctcatcgtcg
      301 tagaagtcaa tcgtggcttg cactgggaaa ctggccagta cttgctcccc ggaactccgc
      361 aggtattctt gacgctttga catgggtaaa tagactctta caggatgctg aaacccaaag
      421 gctccctttg atccaaaagt gtgttggtca gccctaggtt caagcggcct tccagttctg
      481 gtcagctcag catctccagg tgtctggatc caaggtcgcc acggcgcggg gctctccggc
      541 ccgcgcggtg gcggaggcct ccagggagcg tccccggggc agtgacagcc gcgcccccgc
      601 gccttccggg ctccggccgc cgcttcgggc tccatggtgc ctgctttttt gtacaaactt
      661 gtgatccttg aattcgaatc gatgggatcc tgcaaaaaga acaagtagct tgtattctat
      721 agtgtcacct aaatcaagct tgct
//
