LOCUS       sequence-94992-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR4_pCSdest
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(419..443)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(497..515)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(498..515)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gactcactat agttctagag gctcgagagg atcaccactt tgtacaagaa agctgggttc
       61 taggttgatg ggcttgcgct tatctgcttg tcccttctcc catctgattt cttgtggagc
      121 ccatggaggt aaagttggta tttccaaatc agacattttg cccaaaattg ctgcccctct
      181 ctggttatga ttgtggggct ggcaagtcca gaatccgtag gagaagctgg caggctgaaa
      241 ctcaggcagg aaacgatgtt gcagacttta ggcagaattt tttcttctct gtggagagga
      301 gaagctgagg caggatcagg aagcggggaa gtagagtgca atgctggtga agcggcatca
      361 ctgtctgggg taaatatccg gggttcatca tctctcgtca agatgattaa cgacatggtg
      421 cctgcttttt tgtacaaact tgtgatcctt gaattcgaat cgatgggatc ctgcaaaaag
      481 aacaagtagc ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc
      541 agaatggcgg ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc
      601 gcccatttac gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg
      661 gggatgtact tggcagccat cgcgggccat ttaccgccat tgacgtc
//
