LOCUS       sequence-95-expo        7610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_18
FEATURES             Location/Qualifiers
     source          1..7610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             527..547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(637..659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5050..5072)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5172..5191
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5269..5287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5355..5459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5460..5528,5529..6320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5678..5697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6491..7079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6980..6999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7233..7250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7367..7388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7403..7408,7409..7426,7427..7433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7441..7457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7446..7468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7465..7481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7465..7481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc cttggtcgac tctagaggat ccccggggat tggccaaagg
       61 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
      121 gcttggctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca
      181 gcatccgcac tggcagtctc cgaaccggcc tgcaggatcg attttttgca aattacgagc
      241 gttgtagggg gcggagcgat aggtcctata ggttttggta tatcatcatt cattcattca
      301 ttggtacatt catttaccca ccttcctctt tctgagcttc tctggagttc tgtgcttcct
      361 ttttccctta tctttatact gtaattttta actttcaggc attgattgga tccccgggat
      421 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      481 gaggaccctt gcttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
      541 taaggtaccg gtgggtgaag accagaaaca gcacctcgaa ctgagccgcg atattgccca
      601 gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt ttacaacgtc gtgactggga
      661 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgaaggt aagtttaaac
      781 agatccatac taactaactt gttctgacat aattttcagc ttgaatggcg aatggcgctt
      841 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
      901 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
      961 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     1021 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     1081 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     1141 ttacggccag gtaagtttaa ttaagttgat actaactaac aagatctgat taattttcag
     1201 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
     1261 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg
     1321 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
     1381 gatttccatg ttgccactcg ctttaatgat gatttctccc gcgctgtact ggaggctgaa
     1441 gtccaggtaa gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagatg
     1501 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
     1561 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
     1621 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
     1681 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     1741 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     1801 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     1861 atggatgagc agacgatggt gcaggatgta agtttaaact attcgttact aactaacttt
     1921 aaacatttaa attttcagat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     1981 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     2041 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     2101 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     2161 cacccgagtg tgatcatctg gtcgctgggg aatggtaagt ttaaacagtt gaatactaac
     2221 taacggagat ctttgaaatt ttcagaatca ggccacggcg ctaatcacga cgcgctgtat
     2281 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
     2341 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
     2401 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
     2461 ctgattcttt gcgaggtaag tttaaacaga actctactaa ctaacacatt agatcctaat
     2521 tttcagtacg ctcacgcgat gggcaacagt cttggcggtt tcgctaaata ctggcaggcg
     2581 tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg
     2641 attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg
     2701 ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca
     2761 gcgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt caggctgacg
     2821 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
     2881 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
     2941 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
     3001 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
     3061 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcatggcag
     3121 cagtggaggt aagtttaaac aagatcctac taactaactc tacattgatg aattttcaga
     3181 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
     3241 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
     3301 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
     3361 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
     3421 gaccctaacg cctgggtcgg taagtttaaa caaagttgta ctaactaacg aagatcttga
     3481 taattttcag aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
     3541 tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat
     3601 caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
     3661 gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg
     3721 aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa
     3781 aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
     3841 atgtagtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagtacc
     3901 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     3961 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     4021 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     4081 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
     4141 caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg
     4201 ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     4261 agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     4321 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     4381 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     4441 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     4501 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     4561 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     4621 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     4681 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     4741 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     4801 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     4861 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     4921 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     4981 ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     5041 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     5101 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     5161 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5221 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     5281 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5341 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5401 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5461 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5521 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5581 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5641 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5701 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5761 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5821 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5881 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5941 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6001 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6061 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6121 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6181 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6241 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6301 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6361 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6421 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6601 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     6901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7141 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7201 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7261 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7321 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7381 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7441 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
     7501 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
     7561 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
