LOCUS       sequence-95001-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TMEM50B_pCSdest
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(542..566)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(620..638)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(621..638)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaggtcc atagctcttc ggttcttcca aatttgtaga tcagagtgct aaaaaatata
      121 agtgcatttt gaaaaaacac agctagtccc ggataaacat cagtattttg ggtaacatat
      181 gcaccaaaaa gaatccacat ggaagcaata agtgacccaa acatcaacat gaaaccaatg
      241 aaaagccaaa ctcgagcacc tgttcttcct aaacagccgc tttcatagct atcacctctc
      301 acctgagcat tggatacagc atttatcatg aagaaagcca atgtggaaaa tacaccacat
      361 gtgtgaaagg catggttcaa ctgttctggc ttaggataca ccacagctgc atcaatcatt
      421 atccaccagc ctgtaaaaaa caatatacct gcgacaacag atgccacagc atttcttctc
      481 tcactccagt caatacattc acattctggc caacgaaaat tatctaggaa gcctgccatg
      541 gtgcctgctt ttttgtacaa acttgtgatc cttgaattcg aatcgatggg atcctgcaaa
      601 aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc gacgtcccca
      661 ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt aggcacgcct
      721 accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc attgacgtca
      781 atgggggatg tac
//
