LOCUS       sequence-95005-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PIGP_pCSdest
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             275..283
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(539..563)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(617..635)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(618..635)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gactcactat agttctagag gctcgagagg atcaccactt tgtacaagaa agctgggttc
       61 tagtttttgg tgtaaagttc tttggctgca agaaagaaca tttggtttac ttcactaata
      121 gaaatatctc ttaaggctgg aatggcctcc tcttggtatt tcttctgctg ttgatttttt
      181 gcatagttat ctgtgattgt atggatggag tcgagtggag aggtactcat catgttaatc
      241 ccaaacaaga gcacgtagcc aattactata gcaataagga ggtagacagg taatgcaact
      301 gcccaatatt tttgaggcca ataggttaaa cctaaagagt ttagccaaga ttcaggaata
      361 aaggcccaca cgaggtaaag tatgaagcca aattgggagc ttaagaaaag aacaaagcca
      421 taaatcgctc tttctggcaa tggcgacggt gaattttcca ccatttttcc tggggcttta
      481 gacaatctgt ggaaaaggaa cacaatcagc gtcagcgatg tgctccgtgg caccatggtg
      541 cctgcttttt tgtacaaact tgtgatcctt gaattcgaat cgatgggatc ctgcaaaaag
      601 aacaagtagc ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc
      661 agaatggcgg ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc
      721 gcccatttac gtcaatggaa cgcccatttg cgtcattg
//
