LOCUS       sequence-95007-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR8_pCSdest
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(176..200)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(254..272)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(255..272)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gactcactat agttctagag gctcgagagg atcaccactt tgtacaagaa agctgggttc
       61 tattccagaa ctttcttgat ttccaaggtt tgtagtaggt gcttaacaaa tgccatcttg
      121 aatgaatgaa gaccctttct cacagtaaca aagttgggtc caggctcctt catggtgcct
      181 gcttttttgt acaaacttgt gatccttgaa ttcgaatcga tgggatcctg caaaaagaac
      241 aagtagcttg tattctatag tgtcacctaa atcaagcttg ctccgacgtc cccaggcaga
      301 atggcggttc cctaaacgag cattgcttat atagacctcc cattaggcac gcctaccgcc
      361 catttacgtc aatggaacgc ccatttgcgt cattgcccct ccccattgac gtcaatgggg
      421 atgtacttgg cagccatcgc gggccattta ccgccattga cgtcaatggg agtactgcca
      481 atgtaccctg gcgtacttcc aatagtaatg tacttgccaa gttactatta atagatattg
      541 atgtactgcc aagtgggcca tttaccgtca ttgacgtcaa tagggggcgt gagaacggat
      601 atgaatgggc aatgagccat cccattgacg tcaatggtgg gtggtcctat tgacgtcaat
      661 gggcattgag ccaggcgggc catttaccgt aattgacgtc aat
//
