LOCUS       sequence-95014-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf119_pCSdest
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(251..275)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(329..347)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(330..347)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctattgtg ctgcttggac gcgccggggc ccgagtcttc gtggatcggc caggccagga
      121 gtgaaaggtc tgggagttct tccggccaag agcacccgaa gagccactgg gatcagaaat
      181 gcgccccggt ggaccccaaa ccctgcagcc gggggcgggg ggtgtcgtgg agtgtccgct
      241 cgccccatgg tgcctgcttt tttgtacaaa cttgtgatcc ttgaattcga atcgatggga
      301 tcctgcaaaa agaacaagta gcttgtattc tatagtgtca cctaaatcaa gcttgctccg
      361 acgtccccag gcagaatggc ggttccctaa acgagcattg cttatataga cctcccatta
      421 ggcacgccta ccgcccattt acgtcaatgg aacgcccatt tgcgtcattg cccctcccca
      481 ttgacgtcaa tggggatgta cttggcagcc atcgcgggcc atttaccgcc attgacgtca
      541 atgggagtac tgccaatgta ccctggcgta cttccaatag taatgtactt gccaagttac
      601 tattaataga tattgatgta ctgccaagtg ggccatttac cgtcattgac gtcaataggg
      661 ggcgtgagaa cggatatgaa tgggcaatga gccatcccat tgacgtcaat ggtgggtggt
      721 cctattgacg tcaatgggca ttgagccagg cgggccattt accgtaattg acgtcaat
//
