LOCUS       sequence-95016-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf49_pCSdest
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(305..329)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(383..401)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(384..401)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctacaaat tcttctggga acttcttgtc ccacatgttc cgcaaaccaa tatccttttc
      121 tcttctgatc tcttttcctc ttccacggct gcctctaaaa ggatcagcac cttccatgca
      181 gtagcccatt cttcaacatt gagagtcatg attctgaaag caccaaaggt ggtagatgat
      241 gaggttgtgg aatttaaggc catgggacaa attggcatca taagctgcct gacatgatgc
      301 atggtgcctg cttttttgta caaacttgtg atccttgaat tcgaatcgat gggatcctgc
      361 aaaaagaaca agtagcttgt attctatagt gtcacctaaa tcaagcttgc tccgacgtcc
      421 ccaggcagaa tggcggttcc ctaaacgagc attgcttata tagacctccc attaggcacg
      481 cctaccgccc atttacgtca atggaacgcc catttgcgtc attgcccctc cccattgacg
      541 tcaatgggga tgtacttggc agccatcgcg ggccatttac cgccattgac gtcaatggga
      601 gtactgccaa tgtaccctgg cgtacttcca atagtaatgt acttgccaag ttactattaa
      661 tagatattga tgtactgcca agtgggccat ttaccgtcat tgacgtcaat agggggcgtg
      721 agaacggata tgaatgggca atgagccatc ccattgacgt caat
//
