LOCUS       sequence-95029-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  detection of activity of ubiquitous calpains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Stockholm D, Bartoli M, Sillon G, Bourg N, Davoust J, Richard I
  TITLE     Imaging calpain protease activity by multiphoton FRET in living 
            mice.
  JOURNAL   J Mol Biol. 2005 Feb 11;346(1):215-22. Epub 2004 Dec 16.
  PUBMED    15663939
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-02-11"; volume: "346(1)"; pages: "215-22. Epub 
            2004 Dec 16"
COMMENT     Sequence Label: pCMV-calpainsensor
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(40..42,43..45,46..756)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(85..106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(346..365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     693..714
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
      241 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
      661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      721 gccgggatca ctctcggcat ggacgagctg tacaagtccg gaagcggcca gcaggaggtg
      781 tatggtgcga tgcccaggga tggctcaggg gatccaccgg tcgccac
//
